Fozol Ovi

Lab management: Compliance and management of Animal Biosafety Level-2 (ABSL-2) facilities

Microbiology:

1. Avian Pathogenic Escherichia coli (APEC), Salmonella Enterica, Salmonella Typhimurium investigation in Layer hen

2. In vitro and in vivo probiotics intervention (Lactobacillus reuteri, and L. acidophilus) against salmonellosis

3. Embryo lethality assay, in-ovo inoculation and experimental infection model with APEC in layer chicken

4. Antibiotic susceptibility assay (CLSI-standard, 33rd edition)

Molecular biology:

1. Gut and litter microbiome analysis (16s rRNA sequencing), and expression of inflammatory cytokines in gut (2-ΔΔCT)

2. Multi-locus sequence typing and whole genome sequencing of E. coli (Oxford Nanopore sequencing)

Immuno-serology:

1. Adhesion invasion assay of E. coli in chicken macrophage.

2. Estimation of immune cell population in response to APEC infection (CD45+, CD4+, CD8+, CD41/61, γδTcells)

3. Antibody titer estimation by Enzyme linked immunosorbent assay (ELISA).

Nutrition:

1. Feed formulation, nutrient digestibility, phytase activity phytase & xylanase enzyme supplementation recovery in feed.

2. Live performance evaluation of broiler, FCR, Avg daily gain, Bone breaking strength, tibial ash content

3. Determining the digestible lysin: arginine ratio for Ross-708 broilers using linear and quadratic broken line regression

Feed manufacturing:

1. Compliance of research feed mill in Charles C. Miller Jr. Poultry Research & Education Center

2. Determination of the nutrient composition of feed ingredient by Near-infrared spectroscopy (NIR)

3. Evaluating the effects of feed particle size, feed form, conditioning temperature, and whole grain inclusion on broilers

4. Mycotoxins monitoring in the bulk feed ingredients (corn and soybean meal)

5. Inventory and supply chain management of micro-ingredients

Processing and meat quality:

1. USDA compliance for processing plant, HACCP, GMP, and microbial contamination prevention of poultry

2. Investigation of breast myopathy, coloration, and cooking loss with different lysine levels in poultry diet

• Breeder and Hatchery Business of Charoen Pokphand (CP) Bangladesh
• Grand-parent breeding: Flock scheduling, chick receive and brooding, male line management, bird selection, and sperm quality evaluation
• -Parent hatchery management, hatch-out scheduling, hatch residue analysis, vent sexing and inovo vaccination Biosecurity designing, vaccine program development, antibody titer evaluation